不同条件延缓未受精卵激活对鱼类细胞电融合的影响     

吴滟  傅洪拓  龚永生  何新龙  熊贻伟 《广东海洋大学学报》2006,26(6):1-4

泥鳅未受精卵先在合成卵巢液、郝氏液中分别浸泡15、20、30min,然后再受精孵化,统计发育情况,结果表明,合成卵巢液可有效延缓未受精卵遇水后被激活的时间,郝氏液对延缓未受精卵被激活也有一定作用,但效果比合成卵巢液差。用合成卵巢液配制不同浓度胰酶处理受体卵进行细胞电融合实验,胰酶质量浓度为5 mg/mL时,融合卵发育至囊胚期的比例比对照组(郝氏液)高2%,统计分析表明该差异是显著的(P<0.05);但发育至原肠期及孵出期的比例则与对照组无显著差异。胰酶质量浓度为2和1 mg/mL时,融合卵发育到各阶段的比例均低于对照组,统计分析表明两者的差异是显著的(P<0.05)。研究表明,溶液种类、胰酶浓度等对电融合卵的发育有明显的影响,但延缓受体卵的激活时间并不能提高电融合卵的出苗率。用郝氏液配制的2 mg/mL的胰酶溶液处理受体卵,可获得较高比例的融合鱼。  相似文献   

Fast tidal cycling and the origin of life     

Richard Lathe 《Icarus》2004,168(1):18-22

Replicating prebiotic polymers are thought to predate the emergence of true life-forms. The initial mode of replication, a prerequisite for Darwinian selection, is unknown, but demands an explanation based on local physicochemistry. Dual consideration of the conditions of the early terrestrial surface, with the unusual physicochemical properties of nucleic acids like DNA, could explain the emergence of nucleic acids as key biomolecules. The early impact that produced the Moon, and fast terrestrial rotation, subjected coastal areas 3.9 Ga ago to rapid tidal flooding (dilution) and drying (concentration), with a likely periodicity in the range of 2-6 h, and could have provided a driving force for cyclic replication of early biomolecules. Such a mechanism applies only to molecules capable of association/polymerization at high salt concentration, and of dissociation at low salinity. Nucleic acids meet these criteria. It is suggested that tidal cycling, resembling the polymerase chain reaction (PCR) mechanism, could only replicate and amplify DNA-like polymers. This mechanism suggests constraints on the evolution of extra-terrestrial life.  相似文献   

CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES     

郭华荣  张士璀  李红岩  童裳亮  相建海 《中国海洋湖沼学报》2002,20(3):226-231

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.  相似文献   

Readout Techniques for Drift and Low Frequency Noise Rejection in Infrared Arrays     

Gert Finger  Reinhold J. Dorn  Alan W. Hoffman  Hamid Mehrgan  Manfred Meyer  Alan F. M. Moorwood  Joerg Stegmeier 《Experimental Astronomy》2002,14(2):117-127

Three different methods are presented to subtract thermal drifts and low-frequency noise from the signal of infrared array. The first is dead pixels with open Indium bumps, the second is reference output as implemented on the Hawaii2 multiplexer, and the third is dark pixels to emulate reference cells having a capacity connected to the gate of the unit cell field-effect transistor (FET). The third method is the most effective and yields a reduction in readout noise from15.4–9.4 erms. A novel method will be described to extend this readout technique to the Aladdin 1 K × 1 K InSb array. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

一种智能电话语音报时系统     

白燕  和康元 《时间频率学报》1999,22(2):110-113

介绍了电话语音报时系统的设计思路及原理 ,并阐述了系统硬件及通讯接口设计。该系统硬件结构简单、报时准确 ,可以满足一般用户的需求。  相似文献   

川青藏地区的地震活动及震源特征   总被引：4，自引：0，他引：4       下载免费PDF全文

高原  马林 《地震地质》1998,20(4):126-422

在分析区域地震活动和构造环境及利用ＧＤＳＮ的宽频带波形资料分析震源破裂特征的基础上,结合余震资料,讨论了１９８８～１９９０年发生在四川和青海的９次强震的震源破裂和余震分布特征,分析了四川、青海、西藏相邻区域内的地震能量迁移,并用单键群分析方法对１９７０年以来的地震活动总体图象进行了研究。结果表明,这些地震的震源性质与构造应力环境相符,但青海地震的震源都具有较大的逆掩分量,与过去的走滑震源性质不同,而四川地震的震源Ｐ轴不接近水平方向与当地的“Ｙ”字形复杂构造有关  相似文献   

胜利油田火山岩单个包裹体碳同位素   总被引：10，自引：1，他引：9       下载免费PDF全文

赫英 《地质科学》1998,33(3):380-383

近年来,在我国东部陆续发现了一些二氧化碳气藏,对其成因,不少学者有过讨论, 得出许多重要认识^①。  相似文献   

地壳浅部导电性矿物的一种新的压溶机制：应力原电池过程   总被引：3，自引：0，他引：3  

李和平 《矿物学报》1998,18(1):80-83

以黄铁矿为例,通过导电性矿物的力学-电化学相互作用实验,得出：导电性矿物的电极电位与弹性轴压应力是较好的负线性相关关系。以此为基础,通过电化学理论分析,得出：地壳浅部与溶液接触的导电性矿物颗粒内部存在各种各样的应力原电池,通过应力原电池过程,压溶作用得以实现。导电性矿物应力原电池引起压涛作用的发现,无疑对诸如构造地球化学、地电化学甚至湿法冶金等领域的有关研究具有重要意义。  相似文献   

新矿物(19931～199312)     

黄蕴慧  蔡剑辉  曹亚文 《岩石矿物学杂志》1998,(2)

本文以表格形式列举了１９９３年１月至１９９３年１２月经《国际矿物学协会（ＩＭＡ）新矿物与矿物命名委员会（ＣＮＭＭＮ）》批准并在各国矿物学杂志上发表的４２个新矿物的中文英文名称、化学式及其他数据。数据依次为：矿物的晶系、空间群、晶胞参数、主要粉晶数据（Ｉ、ｈｋｌ）、物理性质（晶形、颜色、条痕、光泽、解理、发光、硬度、密度等）、光学性质（一、二轴晶,正、负光性,折光率,反射率,双反射,２Ｖ,色散,吸收性,多色性等）、产状、共生矿物等。  相似文献   

香港大屿山残坡积土的残余强度试验研究   总被引：8，自引：1，他引：7  

戴福初  李焯芬 《工程地质学报》1998,6(3):223-229

粘性土的残余强度是边坡稳定性评价、桩基与土的相互作用机理研究及填土边坡设计中的重要参数。本文在综述大量文献的基础上,结合香港大屿山火山岩风化残坡积土的残余强度试验研究,分析了残余强度的测试方法和影响残余强度的因素。研究结果表明,残余强度与有效法向应力间具有明显的非线性关系;与单剪测试结果相比,多级剪测试结果明显偏高。  相似文献